Free and immobilized glucose isomerase from Streptomyces phaeochromogenes.
نویسندگان
چکیده
Properties were determined of the glucose isomerase from Streptomyces phaeochromogenes NRRL B-3559. The enzyme exhibited a temperature optimum of 80 C and a pH optimum of about 8. The effect of various buffers on activity of the enzyme and the optimum pH were studied. Michaelis constants for glucose and Mg(2+) were 0.25 and 0.025 m, respectively. Co(2+) enhanced enzyme activity. A functional polyacrylamide-entrapped glucose isomerase was prepared. The conditions for entrapment and use of the bound enzyme were examined.
منابع مشابه
Determination of Kinetic Parameters for Catalytic Isomerization of Glucose to Fructose by immobilized Glucose Isomerase in an Aqueous-Ethanol Medium
Catalytic isomerization of glucose to fructose by immobilized glucose isomerase in an aqueous-ethanol medium was studied. Using Michaelis-Menten equation and Haldane relationship, the main kinetic parameters were determined: Kmf, Vmf, Kmr, Vmr. By comparing the aqueous-ethanol medium with an aqueous solution, the measured values of Kmr and Kmf were increased in the aqueous-ethanol solution, whi...
متن کاملDetermination of Kinetic Parameters for Catalytic Isomerization of Glucose to Fructose by immobilized Glucose Isomerase in an Aqueous-Ethanol Medium
Catalytic isomerization of glucose to fructose by immobilized glucose isomerase in an aqueous-ethanol medium was studied. Using Michaelis-Menten equation and Haldane relationship, the main kinetic parameters were determined: Kmf, Vmf, Kmr, Vmr. By comparing the aqueous-ethanol medium with an aqueous solution, the measured values of Kmr and Kmf were increased in the aqueous-ethanol solution, whi...
متن کاملOptimization of HFDS Production from Date Syrup (RESEARCH NOTE)
Fructose is utilized as a common sweetener in many food industries. Today, a large amount of HFCS (high fructose corn syrup, an equilibrium mixture of glucose and fructose) is produced by glucose isomerase, immobilized enzymes from corn starch. There are a lot of date palms in Iran. Date is a favorable source of carbohydrates, and a suitable source for HFDS (high fructose date syrup) production...
متن کاملImmobilized Trienzymatic System with Enhanced Stabilization for the Biotransformation of Lactose.
The use of ketohexose isomerases is a powerful tool in lactose whey processing, but these enzymes can be very sensitive and expensive. Development of immobilized/stabilized biocatalysts could be a further option to improve the process. In this work, β-galactosidase from Bacillus circulans, l-arabinose (d-galactose) isomerase from Enterococcus faecium, and d-xylose (d-glucose) isomerase from Str...
متن کاملSelective isolation of acidophilic streptomyces strains for glucose isomerase production.
Approximately 260 Streptomyces strains were isolated from neutral pH farmland soil and evaluated for their ability to produce glucose isomerase. The number of acidophilic Streptomyces organisms growing at pH 4.0 was low, i.e., 10 organisms per g of soil. All of the isolates showed glucose isomerase activity when they were grown in a medium containing d-xylose, an inducer for glucose isomerase. ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied microbiology
دوره 21 4 شماره
صفحات -
تاریخ انتشار 1971